Abstract
The giant tiger prawn (Penaeus monodon) is a decapod crustacean widely reared for human consumption. Currently, viruses of two distinct lineages of parvoviruses (PVs, familyParvoviridae; subfamilyHamaparvovirinae) infect penaeid shrimp. Here, a PV was isolated and cloned from VietnameseP. monodonspecimens, designatedPenaeus monodonmetallodensovirus (PmMDV). This is the first member of a third divergent lineage shown to infect penaeid decapods. PmMDV has a transcription strategy unique among invertebrate PVs, using extensive alternative splicing and incorporating transcription elements characteristic of vertebrate-infecting PVs. The PmMDV proteins have no significant sequence similarity with other PVs, except for an SF3 helicase domain in its nonstructural protein. Its capsid structure, determined by cryoelectron microscopy to 3-Å resolution, has a similar surface morphology toPenaeus stylirostrisdensovirus, despite the lack of significant capsid viral protein (VP) sequence similarity. Unlike other PVs, PmMDV folds its VP without incorporating a βA strand and displayed unique multimer interactions, including the incorporation of a Ca2+cation, attaching the N termini under the icosahedral fivefold symmetry axis, and forming a basket-like pentamer helix bundle. While the PmMDV VP sequence lacks a canonical phospholipase A2 domain, the structure of an EDTA-treated capsid, determined to 2.8-Å resolution, suggests an alternative membrane-penetrating cation-dependent mechanism in its N-terminal region. PmMDV is an observed example of convergent evolution among invertebrate PVs with respect to host-driven capsid structure and unique as a PV showing a cation-sensitive/dependent basket structure for an alternative endosomal egress.
Funder
Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada
HHS | NIH | National Institute of Biomedical Imaging and Bioengineering
Southeastern Consortium for Microscopy of MacroMolecular Machines
Publisher
Proceedings of the National Academy of Sciences
Reference91 articles.
1. Reorganizing the family Parvoviridae: a revised taxonomy independent of the canonical approach based on host association
2. ICTV virus taxonomy profile: Parvoviridae;Cotmore;J. Gen. Virol.,2019
3. A Viral Phospholipase A2 Is Required for Parvovirus Infectivity
4. Parvoviral virions deploy a capsid-tethered lipolytic enzyme to breach the endosomal membrane during cell entry
5. P. Tijssen , M. Bergoin , “Densoviruses: A highly diverse group of arthropodparvoviruses” in Insect Virology, S. Asgari , K. N. Johnson , Eds. (Caister Academic Press, Norwich, UK, 2010), pp. 57–90.
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