Cosmcis an X-linked inflammatory bowel disease risk gene that spatially regulates gut microbiota and contributes to sex-specific risk

Author:

Kudelka Matthew R.,Hinrichs Benjamin H.,Darby Trevor,Moreno Carlos S.,Nishio Hikaru,Cutler Christopher E.,Wang Jianmei,Wu Huixia,Zeng Junwei,Wang Yingchun,Ju Tongzhong,Stowell Sean R.,Nusrat Asma,Jones Rheinallt M.,Neish Andrew S.,Cummings Richard D.

Abstract

Inflammatory bowel disease (IBD) results from aberrant immune stimulation against a dysbiotic mucosal but relatively preserved luminal microbiota and preferentially affects males in early onset disease. However, factors contributing to sex-specific risk and the pattern of dysbiosis are largely unexplored. Core 1 β3GalT-specific molecular chaperone (Cosmc), which encodes an X-linked chaperone important for glycocalyx formation, was recently identified as an IBD risk factor by genome-wide association study. We deletedCosmcin mouse intestinal epithelial cells (IECs) and found marked reduction of microbiota diversity in progression from the proximal to the distal gut mucosa, but not in the overlying lumen, as seen in IBD. This loss of diversity coincided with local emergence of a proinflammatory pathobiont and distal gut restricted pathology. Mechanistically, we found that Cosmc regulates host genes, bacterial ligands, and nutrient availability to control microbiota biogeography. Loss of oneCosmcallele in males (IEC-Cosmc-/y) resulted in a compromised mucus layer, spontaneous microbe-dependent inflammation, and enhanced experimental colitis; however, females with loss of one allele and mosaic deletion ofCosmcin 50% of crypts (IEC-Cosmc+/−) were protected from spontaneous inflammation and partially protected from experimental colitis, likely due to lateral migration of normal mucin glycocalyx from WT cells over KO crypts. These studies functionally validateCosmcas an IBD risk factor and implicate it in regulating the spatial pattern of dysbiosis and sex bias in IBD.

Funder

HHS | NIH | National Cancer Institute

HHS | NIH | National Institute of General Medical Sciences

HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases

Division of Cancer Prevention, National Cancer Institute

Division of Intramural Research, National Institute of Allergy and Infectious Diseases

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

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