Author:
Zhu Shoujun,Yang Qinglai,Antaris Alexander L.,Yue Jingying,Ma Zhuoran,Wang Huasen,Huang Wei,Wan Hao,Wang Joy,Diao Shuo,Zhang Bo,Li Xiaoyang,Zhong Yeteng,Yu Kuai,Hong Guosong,Luo Jian,Liang Yongye,Dai Hongjie
Abstract
Fluorescence imaging multiplicity of biological systems is an area of intense focus, currently limited to fluorescence channels in the visible and first near-infrared (NIR-I; ∼700–900 nm) spectral regions. The development of conjugatable fluorophores with longer wavelength emission is highly desired to afford more targeting channels, reduce background autofluorescence, and achieve deeper tissue imaging depths. We have developed NIR-II (1,000–1,700 nm) molecular imaging agents with a bright NIR-II fluorophore through high-efficiency click chemistry to specific molecular antibodies. Relying on buoyant density differences during density gradient ultracentrifugation separations, highly pure NIR-II fluorophore-antibody conjugates emitting ∼1,100 nm were obtained for use as molecular-specific NIR-II probes. This facilitated 3D staining of ∼170-μm histological brain tissues sections on a home-built confocal microscope, demonstrating multicolor molecular imaging across both the NIR-I and NIR-II windows (800–1,700 nm).
Publisher
Proceedings of the National Academy of Sciences
Cited by
230 articles.
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