Author:
Lee Kyung S.,Grenfell Tallessyn Z.,Yarm Frederic R.,Erikson Raymond L.
Abstract
Members of the polo subfamily of protein kinases play pivotal roles in cell proliferation. In addition to the kinase domain, polo kinases have a strikingly conserved sequence in the noncatalytic domain, termed the polo-box. The function of the polo-box is currently undefined. The mammalian polo-like kinase Plk is a functional homologue ofSaccharomyces cerevisiaeCdc5. Here, we show that Plk localizes at the spindle poles and cytokinetic neck filaments. Without impairing kinase activity, a conservative mutation in the polo-box disrupts the capacity of Plk to complement the defect associated with acdc5–1temperature-sensitive mutation and to localize to these subcellular structures. Our data provide evidence that the polo-box plays a critical role in Plk function, likely by directing its subcellular localization.
Publisher
Proceedings of the National Academy of Sciences
Cited by
223 articles.
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