Neural induction by the node and placode induction by head mesoderm share an initial state resembling neural plate border and ES cells
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Published:2017-12-19
Issue:2
Volume:115
Page:355-360
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ISSN:0027-8424
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Container-title:Proceedings of the National Academy of Sciences
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language:en
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Short-container-title:Proc Natl Acad Sci USA
Author:
Trevers Katherine E.,Prajapati Ravindra S.,Hintze Mark,Stower Matthew J.,Strobl Anna C.,Tambalo Monica,Ranganathan Ramya,Moncaut Natalia,Khan Mohsin A. F.,Stern Claudio D.,Streit Andrea
Abstract
Around the time of gastrulation in higher vertebrate embryos, inductive interactions direct cells to form central nervous system (neural plate) or sensory placodes. Grafts of different tissues into the periphery of a chicken embryo elicit different responses: Hensen’s node induces a neural plate whereas the head mesoderm induces placodes. How different are these processes? Transcriptome analysis in time course reveals that both processes start by induction of a common set of genes, which later diverge. These genes are remarkably similar to those induced by an extraembryonic tissue, the hypoblast, and are normally expressed in the pregastrulation stage epiblast. Explants of this epiblast grown in the absence of further signals develop as neural plate border derivatives and eventually express lens markers. We designate this state as “preborder”; its transcriptome resembles embryonic stem cells. Finally, using sequential transplantation experiments, we show that the node, head mesoderm, and hypoblast are interchangeable to begin any of these inductions while the final outcome depends on the tissue emitting the later signals.
Funder
RCUK | Biotechnology and Biological Sciences Research Council
Action on Hearing Loss
HHS | NIH | National Institute on Deafness and Other Communication Disorders
HHS | NIH | National Institute of Dental and Craniofacial Research
Diana Trebble Studentship
RCUK | Medical Research Council
EC | FP7 | FP7 Ideas: European Research Council
Publisher
Proceedings of the National Academy of Sciences
Subject
Multidisciplinary
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