Author:
Sievert Christian,Nieves Lizbeth M.,Panyon Larry A.,Loeffler Taylor,Morris Chandler,Cartwright Reed A.,Wang Xuan
Abstract
Microbial production of fuels and chemicals from lignocellulosic biomass provides promising biorenewable alternatives to the conventional petroleum-based products. However, heterogeneous sugar composition of lignocellulosic biomass hinders efficient microbial conversion due to carbon catabolite repression. The most abundant sugar monomers in lignocellulosic biomass materials are glucose and xylose. Although industrialEscherichia colistrains efficiently use glucose, their ability to use xylose is often repressed in the presence of glucose. Here we independently evolved threeE. colistrains from the same ancestor to achieve high efficiency for xylose fermentation. Each evolved strain has a point mutation in a transcriptional activator for xylose catabolic operons, either CRP or XylR, and these mutations are demonstrated to enhance xylose fermentation by allelic replacements. Identified XylR variants (R121C and P363S) have a higher affinity to their DNA binding sites, leading to a xylose catabolic activation independent of catabolite repression control. Upon introducing these amino acid substitutions into theE. coliD-lactate producer TG114, 94% of a glucose–xylose mixture (50 g⋅L−1each) was used in mineral salt media that led to a 50% increase in product titer after 96 h of fermentation. The two amino acid substitutions in XylR enhance xylose utilization and release glucose-induced repression in differentE. colihosts, including wild type, suggesting its potential wide application in industrialE. colibiocatalysts.
Funder
HHS | NIH | National Human Genome Research Institute
Arizona State University
Publisher
Proceedings of the National Academy of Sciences
Cited by
64 articles.
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