Author:
Cole Russell H.,Tang Shi-Yang,Siltanen Christian A.,Shahi Payam,Zhang Jesse Q.,Poust Sean,Gartner Zev J.,Abate Adam R.
Abstract
Although the elementary unit of biology is the cell, high-throughput methods for the microscale manipulation of cells and reagents are limited. The existing options either are slow, lack single-cell specificity, or use fluid volumes out of scale with those of cells. Here we present printed droplet microfluidics, a technology to dispense picoliter droplets and cells with deterministic control. The core technology is a fluorescence-activated droplet sorter coupled to a specialized substrate that together act as a picoliter droplet and single-cell printer, enabling high-throughput generation of intricate arrays of droplets, cells, and microparticles. Printed droplet microfluidics provides a programmable and robust technology to construct arrays of defined cell and reagent combinations and to integrate multiple measurement modalities together in a single assay.
Funder
Office of Extramural Research, National Institutes of Health
DOD | Defense Advanced Research Projects Agency
University of California, San Francisco, Research Allocation Program
Publisher
Proceedings of the National Academy of Sciences
Cited by
143 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献