Author:
Libé-Philippot Baptiste,Michel Vincent,Boutet de Monvel Jacques,Le Gal Sébastien,Dupont Typhaine,Avan Paul,Métin Christine,Michalski Nicolas,Petit Christine
Abstract
Many genetic forms of congenital deafness affect the sound reception antenna of cochlear sensory cells, the hair bundle. The resulting sensory deprivation jeopardizes auditory cortex (AC) maturation. Early prosthetic intervention should revive this process. Nevertheless, this view assumes that no intrinsic AC deficits coexist with the cochlear ones, a possibility as yet unexplored. We show here that many GABAergic interneurons, from their generation in the medial ganglionic eminence up to their settlement in the AC, express two cadherin-related (cdhr) proteins, cdhr23 and cdhr15, that form the hair bundle tip links gating the mechanoelectrical transduction channels. Mutant mice lacking either protein showed a major decrease in the number of parvalbumin interneurons specifically in the AC, and displayed audiogenic reflex seizures.Cdhr15- andCdhr23-expressing interneuron precursors inCdhr23−/−andCdhr15−/−mouse embryos, respectively, failed to enter the embryonic cortex and were scattered throughout the subpallium, consistent with the cell polarity abnormalities we observed in vitro. In the absence of adhesion G protein-coupled receptor V1 (adgrv1), another hair bundle link protein, the entry ofCdhr23- andCdhr15-expressing interneuron precursors into the embryonic cortex was also impaired. Our results demonstrate that a population of newborn interneurons is endowed with specific cdhr proteins necessary for these cells to reach the developing AC. We suggest that an “early adhesion code” targets populations of interneuron precursors to restricted neocortical regions belonging to the same functional area. These findings open up new perspectives for auditory rehabilitation and cortical therapies in patients.
Funder
Agence Nationale de la Recherche
EC | FP7 | FP7 Ideas: European Research Council
BNP Paribas | Fondation BNP Paribas
FAUN Stiftung
LHW-Stiftung
Fondation Agir pour l'Audition
French Ministry of Research
Publisher
Proceedings of the National Academy of Sciences
Cited by
34 articles.
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