Muscle degeneration in chronic massive rotator cuff tears of the shoulder: Addressing the real problem using a graphene matrix

Author:

Saveh Shemshaki Nikoo123,Kan Ho-Man12,Barajaa Mohammed123,Otsuka Takayoshi12ORCID,Lebaschi Amir4,Mishra Neha56,Nair Lakshmi S.12347,Laurencin Cato T.12347ORCID

Affiliation:

1. Connecticut Convergence Institute for Translation in Regenerative Engineering, University of Connecticut Health, Farmington, CT 06030

2. Raymond and Beverly Sackler Center for Biomedical, Biological, Physical, and Engineering Sciences, University of Connecticut Health Center, Farmington, CT 06030

3. Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269

4. Department of Orthopedic Surgery, University of Connecticut Health Center, Farmington, CT 06030

5. Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06269

6. Connecticut Veterinary Medical Diagnostic Laboratory, Storrs, CT

7. Department of Materials Science and Engineering, University of Connecticut, Storrs, CT 06269

Abstract

Massive rotator cuff tears (MRCTs) of the shoulder cause disability and pain among the adult population. In chronic injuries, the tendon retraction and subsequently the loss of mechanical load lead to muscle atrophy, fat accumulation, and fibrosis formation over time. The intrinsic repair mechanism of muscle and the successful repair of the torn tendon cannot reverse the muscle degeneration following MRCTs. To address these limitations, we developed an electroconductive matrix by incorporating graphene nanoplatelets (GnPs) into aligned poly(l-lactic acid) (PLLA) nanofibers. This study aimed to understand 1) the effects of GnP matrices on muscle regeneration and inhibition of fat formation in vitro and 2) the ability of GnP matrices to reverse muscle degenerative changes in vivo following an MRCT. The GnP matrix significantly increased myotube formation, which can be attributed to enhanced intracellular calcium ions in myoblasts. Moreover, the GnP matrix suppressed adipogenesis in adipose-derived stem cells. These results supported the clinical effects of the GnP matrix on reducing fat accumulation and muscle atrophy. The histological evaluation showed the potential of the GnP matrix to reverse muscle atrophy, fat accumulation, and fibrosis in both supraspinatus and infraspinatus muscles at 24 and 32 wk after the chronic MRCTs of the rat shoulder. The pathological evaluation of internal organs confirmed the long-term biocompatibility of the GnP matrix. We found that reversing muscle degenerative changes improved the morphology and tensile properties of the tendon compared with current surgical techniques. The long-term biocompatibility and the ability of the GnP matrix to treat muscle degeneration are promising for the realization of MRCT healing and regeneration.

Funder

HHS | National Institutes of Health

National Science Foundation

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

Reference83 articles.

1. Increased Health Care Costs and Opioid Use in Patients with Anxiety and Depression Undergoing Rotator Cuff Repair

2. Cost analysis and complication rate comparing open, mini-open, and all arthroscopic rotator cuff repair;Marigi E. M.;JSES Rev. Rep. Tech.,2021

3. Immobilization After Rotator Cuff Repair

4. Tissue-engineered tendon constructs for rotator cuff repair in sheep;Novakova S. S.;J. Orthop. Res.,2018

5. Epidemiology of musculoskeletal upper extremity ambulatory surgery in the United States

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3