Enhanced spatial resolution in magnetic resonance imaging by dynamic nuclear polarization at 5 K

Abstract

Significance Although MRI is a powerful method for visualizing features within organisms and materials, the relatively low signal-to-noise ratio (SNR) of the NMR signals that are used to construct an image makes MRI with isotropic spatial resolution below 3.0 μm impractical at room temperature. Here we show that SNR enhancements available from a combination of low temperatures and dynamic nuclear polarization allow MRI with 1.7-μm isotropic resolution. These results may enable informative MRI studies of eukaryotic cells, cell clusters, and small tissue samples.