Evaluation of diuretic activity of gallic acid in normal rats

Abstract

Objective: The present study was designed to evaluate the diuretic activity of Gallic acid in wistar albino rats. Methods: Animals were divided into 4 groups of 6 rat each. Group 1 and group 2 served as control and standard group respectively where as group 3 and 4 were test group which were treated with Gallic acid either at the dose of 25 mg/kg or 50 mglkg orally. All the animals were fasted for 18hr before the experiment. All the animals were given with 2.5 ml/kg of normal saline to impose uniform salt load which was followed by drug treatment for each group. After that animals were kept individually in metabolic cages and observed for amount of urine collected. 24hr Urine samples were collected and analyzed for amount of sodium, potassium and chloride. Results: There was statistically significant increase in urine volume of standard and both the doses of Gallic acid (p<0.001). Urinary excretion of sodium and chloride were increased when compared to control group (p<0.001), Potassium excretion was also increased significantly in standard (p<0.001) and Gallic acid treated group (p<0.05). Conclusion: The result from present study shows that Gallic acid at the dose of 25 mg/kg and 50 mg/kg posses significant diuretic activity.