Isolation of Daphnetin 8-methyl ether from Daphne oleoides and its Anti-bacterial activity

Abstract

The aim of the present work was to isolate and identify secondary metabolites of Daphne oleoides, which was not phytochemically analysed in this important geographical region of the world until now, followed by the determination of anti-bacterial activity. An important plant coumarin daphnetin 8-methyl ether (1)[7-hydroxy8-methoxy coumarin] not previously reported from this species was isolated from the methanolic extract of aerial parts of Daphne oleoides, along with already reported 4-ethoxy benzoic acid (2)and 4-hydroxy benzoic acid(3) by repeated column chromatography. The structures of these compounds were elucidated on the basis of 1H, 13C NMR and MS analysis. The compound 1 was evaluated for its anti-bacterial potential which showed moderate activity.