Author:
SATO H.,OSANAI A.,KAMIYA H.,OBARA Y.,JIANG W.,ZHEN Q.,CHAI J.,UNE Y.,ITO M.
Abstract
Trypanosoma (Herpetosoma) grosi, which naturally parasitizesApodemusspp., can experimentally infect Mongolian jirds (Meriones unguiculatus). Three isolates fromA. agrarius,A. peninsulae, andA. speciosus(named SESUJI, HANTO, and AKHA isolates, respectively) of different geographical origin (AKHA from Japan, and the others from Vladivostok), exhibited different durations of parasitaemia in laboratory jirds (2 weeks for HANTO, and 3 weeks for the others). To assess the genetic background of theseT. grosiisolates, their small (SSU) and large subunit (LSU) ribosomal RNA genes (rDNA) were sequenced along with those of 2 otherHerpetosomaspecies from squirrels. The SSU rDNA sequences of these 3 species along with available sequences of 3 otherHerpetosomatrypanosomes (T. lewisi,T. musculiandT. microti)seemed to reflect well the phylogenetic relationship of their hosts. Three isolates ofT. grosiexhibited base changes at 2–6 positions of 2019-base 18S rDNA, at 5–29 positions of 1817/1818-base 28Sα rDNA, or 1–5 positions of 1557–1559-base 28Sβ rDNA, and none was separated from the other 2 isolates by rDNA nucleotide sequences. Since base changes ofHerpetosomatrypanosomes at the level of inter- and intra-species might occur frequently in specified rDNA regions, the molecular analysis on these regions of rodent trypanosomes could help species/strain differentiation and systematic revision ofHerpetosomatrypanosome species, which must be more abundant than presently known.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Animal Science and Zoology,Parasitology
Reference29 articles.
1. The taxonomic position and evolutionary relationships of Trypanosoma rangeli1Note: Nucleotide sequence data reported in this paper are available in the EMBL, Genbank™ and DDJB databases under the following accession numbers: SsrRNA—Trypanosoma conorhini USP, AJ012411; Trypanosoma leeuwenhoeki CH 250, AJ012412; Trypanosoma minasense LSTM, AJ012413; Trypanosoma rangeli Choachi, AJ012414; T. rangeli Macias, AJ012415; T. rangeli PG, AJ012416; T. rangeli San Agustin, AJ012417; Trypanosoma sp. (Rousettus aegypticus), AJ012418. 5S rRNA and spliced leader (mini-exon)—T. rangeli RGB, AJ012419; T. leeuwenhoeki CH 250, AJ012420.1
2. Ribosomal RNA genes of Trypanosoma brucei: Mapping the regions specifying the six small ribosomal RNAs
3. SPENCER, D. F. , COLLINGS, J. C. , SCHNARE, M. N. & GRAY, M. W. (1987).Multiple spacer sequences in the nuclear large subunit ribosomal RNA gene of Crithidia fasciculata .EMBO Journal 6,1063–1071.
4. WILSON, D. E. & REEDER, D. M. (1993). Mammal Species of the World: a Taxonomic and Geographic Reference , 2nd Edn.Smithsonian Institution,Washington.
5. VIENS, P. (1985).Immunology of nonpathogenic trypanosomes of rodents. In Immunology and Pathogenesis of Trypanosomiasis (ed. Tizard, I. ), pp.201–223.CRC Press,Boca Raton, Florida.
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