Author:
PEREIRA V. R. A.,LORENA V. M. B.,GALVAO DA SILVA A. P.,COUTINHO E. M.,SILVA E. D.,FERREIRA A. G. P.,MIRANDA P.,KRIEGER M. A.,GOLDENBERG S.,SOARES M. B. P.,CORREA-OLIVEIRA R.,GOMES Y. M.
Abstract
In previous studies, we demonstrated that CRA and FRA recombinant proteins, used for diagnosis of Chagas' disease, elicited a humoral immune response in susceptible and resistant mice. To understand better the immune response to these proteins, we have evaluated, the cellular immune response in CRA- and in FRA-immunized BALB/c and C57BL/6 mice. A specific cellular lymphoproliferative response was observed in both strains of mice. Spleen cell cultures mainly from CRA-immunized C57BL/6 and FRA-immunized BALB/c mice produced high levels of IFN-γ, indicating the induction of a Type 1 immune response. Regarding the T cell subsets, CD4+T cells were the major source of IFN-γ in CRA- and FRA-immunized mice. These results suggest that CRA and FRA are important immunogens in inducing a Type 1 immune response and that they may be considered as potential vaccine antigens.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Animal Science and Zoology,Parasitology
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