DNA comparisons ofTrypanosoma evansi(Indonesia) andTrypanosoma bruceispp.

Abstract

SUMMARYTwo clones from separate isolates ofTrypanosoma evansiin Indonesia were found by polymerase chain reaction (PCR) analyses to contain 3 different repeated nuclear DNA sequences ofTrypanosoma bruceispp: the consensus sequence for a highly repetitive 177 base pairs and the gene repeats encoding procyclin and the spliced leader. In addition, the 994 bp minicircle sequence of one of the clones was determined, and PCR amplification primers specific for minicircles ofT. evansiwere identified that do not amplify minicircle sequences in theT. bruceispp. clones tested.