Author:
DALTON J. P.,DAY S. R.,DREW A. C.,BRINDLEY P. J.
Abstract
A novel method for the isolation of schistosome eggs and miracidia
from livers of mice infected with Schistosoma japonicum
or S. mansoni is described. The method employed collagenase B
to
degrade the interstitial matrix of mouse liver tissue,
after which the schistosome eggs were separated from the liver cells by
2
single-step density centrifugations through
Percoll. Using this procedure sufficient quantities of miracidia were
obtained to generate a cDNA library. Southern blot
analysis demonstrated that miracidia isolated by this method were free
from contaminating host DNA.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Animal Science and Zoology,Parasitology
Cited by
134 articles.
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