Molecular and morphological circumscription ofMesocestoidestapeworms from red foxes (Vulpes vulpes) in central Europe

Abstract

SUMMARYHere we examine 3157 foxes from 6 districts of the Slovak Republic in order to determine for the first time the distribution, prevalence and identity ofMesocestodesspp. endemic to this part of central Europe. During the period 2001–2006, an average of 41·9% of foxes were found to harbourMesocestoidesinfections. Among the samples we confirmed the widespread and common occurrence ofM. litteratus(Batsch, 1786), and report the presence, for the first time, ofM. lineatus(Goeze, 1782) in the Slovak Republic, where it has a more restricted geographical range and low prevalence (7%). Using a combination of 12S rDNA, CO1 and ND1 mitochondrial gene sequences together with analysis of 13 morphometric characters, we show that the two species are genetically distinct and can be differentiated by discrete breaks in the ranges of the male and female reproductive characters, but not by the more commonly examined characters of the scolex and strobila. Estimates of interspecific divergence withinMesocestoidesranged from 9 to 18%, whereas intraspecific variation was less than 2%, and phylogenetic analyses of the data showed that despite overlapping geographical ranges, the two commonly reported European species are not closely related, withM. litteratusmore closely allied to North American isolates ofMesocestoidesthan toM. lineatus. We confirm that morphological analysis of reproductive organs can be used to reliably discriminate between these often sympatric species obtained from red foxes.