Further Observations and Experiments on the Cultivation of Entamoeba histolytica from Cysts

Abstract

In a recent paper1, written jointly with Dr P. P. Laidlaw, methods were described for cultivating Entamoeba histolytica, and various other entozoic amoebae of man and monkeys, from the encysted forms. We there showed that cultures of all the species studied can be readily obtained by merely incubating such cysts in any of the media suitable for the cultivation of the corresponding active forms—provided that certain precautions are taken.