Characterization of actin genes inBonamia ostreaeand their application to phylogeny of the Haplosporidia

Abstract

SUMMARYBonamia ostreaeis a protozoan parasite that infects the European flat oysterOstrea edulis, causing systemic infections and resulting in massive mortalities in populations of this valuable bivalve species. In this work, we have characterizedB. ostreaeactin genes and used their sequences for a phylogenetic analysis. Design of different primer sets was necessary to amplify the central coding region of actin genes ofB. ostreae. Characterization of the sequences and their amplification in different samples demonstrated the presence of 2 intragenomic actin genes inB. ostreae, without any intron. The phylogenetic analysis placedB. ostreaein a clade withMinchinia tapetis,Minchinia teredinisandHaplosporidium costaleas its closest relatives, and demonstrated that the paralogous actin genes found inBonamiaresulted from a duplication of the original actin gene after theBonamiaorigin.