Excreted/secreted products of developing Taenia saginata metacestodes

Abstract

SummaryExcretions and secretions (ES) and somatic components of 4, 8, 12 and 16-week-old Taenia saginata metacestodes were biosynthetically radio-isotope labelled by incubating the larvae in the presence of [35S]methionine. Despite their small size, 4-week-old metacestodes produced as much isotope-labelled ES/parasite as older metacestodes, indicating a proportionately greater metabolic activity of the parasite at this age. In situ the 4-week-old metacestodes were surrounded by a marked granulomatous cellular infiltrate which had largely resolved around 8-week-old metacestodes. Examination of the isotope-labelled ES by SDS-PAGE revealed distinct age-specific components from 4- and 12-week-old metacestodes and other ES components which were produced by all the ages of metacestodes examined. In comparison the labelled somatic components were conserved. Antigenic characterization of the ES by immunoprecipitation against a panel of clinically defined bovine sera combined with SDS-PAGE analysis, identified some highly immunogenic parasite products and others which did not elicit an antibody response demonstrable by immunoprecipitation. These components are of interest in relation to the host/parasite relationship, to the construction of diagnostic assays for the detection of T. saginata cysticercosis, and to the immunity that cattle develop against this parasite.