Author:
RODRIGUEZ N.,DE LIMA H.,RODRIGUEZ A.,BREWSTER S.,BARKER D. C.
Abstract
In this paper the Leishmania (Viannia)
braziliensis complex is defined as containing all
species of the actual subgenus
Viannia. Organisms of the L. (V)
braziliensis complex are the causative agents of localized human
cutaneous and
mucocutaneous leishmaniasis in South America, much of Central America and
some areas of North America. In our search
for better species and subspecies diagnostic probes we focused our
research on repetitive DNA, since it provides a greater
number of target sites for hybridization. In this work we report the
isolation and sequencing of a 1·8 kb DNA region,
LbJ38, which is probably tandemly repeated or dispersed at least 4 times
along one chromosome and is naturally present
in L. (V) braziliensis genomic DNA. This
region contains microsatellites and simple repeat DNA sequences and was
isolated by screening a genomic DNA cosmid library with complex- and
species-specific probes. No homology was found
with other Leishmania microsatellite or repetitive DNA. The
utility of this repetitive sequence and primers derived from
it in the identification of L. (V) braziliensis
is demonstrated. As far as we are aware, this is the first report of sequence
characterized repetitive microsatellite and GC rich simple repeat DNA
from the nuclear genome of New World Leishmania.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Animal Science and Zoology,Parasitology
Cited by
27 articles.
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