IgY antibody and human neurocysticercosis: a novel approach on immunodiagnosis usingTaenia crassicepshydrophobic antigens

Abstract

AbstractHuman neurocysticercosis (NCC) is a worldwide neglected disease caused byTaenia soliummetacestode and responsible for various complications and neurological disorders. This study aimed to evaluate the use of specific immunoglobulin Y (IgY) produced by laying hens immunized with a hydrophobic fraction ofTaenia crassicepsmetacestodes (hFTc) in NCC diagnosis. Egg yolk IgY antibodies were fractionated, purified and characterized. Enzyme-linked immunosorbent assay (ELISA) was carried out to evaluate the production kinetics and avidity maturation of anti-hFTcIgY antibodies throughout the IgY obtention process. Antigen recognition tests were carried out by Western blotting and immunofluorescence antibody test using purified and specific anti-hFTcIgY antibodies for detection of parasitic antigens ofT. crassicepsandT. soliummetacestodes. Sandwich ELISA was performed to detect circulating immune complexes formed by IgG and parasitic antigens in human sera. The results showed high diagnostic values (93.2% sensitivity and 94.3% specificity) for immune complexes detection in human sera with confirmed NCC. In conclusion, specific IgY antibodies produced from immunized hens with hFTcantigens were efficient to detectT. soliumimmune complexes in human sera, being an innovative and potential tool for NCC immunodiagnosis.