Life cycle ofCryptosporidium murisin two rodents with different responses to parasitization

Abstract

SUMMARYThis study focuses on mapping the life cycle ofCryptosporidium murisin two laboratory rodents; BALB/c mice and the southern multimammate ratMastomys coucha, differing in their prepatent and patent periods. Both rodents were simultaneously experimentally inoculated with viable oocysts ofC. muris(strain TS03). Animals were dissected and screened for the presence of the parasite using a combined morphological approach and nested PCR (SSU rRNA) at different times after inoculation. The occurrence of first developmental stages ofC. murisin stomach was detected at 2·5 days post-infection (dpi). The presence of Type II merogony, appearing 36 h later than Type I merogony, was confirmed in both rodents. Oocysts exhibiting different size and thickness of their wall were observed from 5 dpi onwards in stomachs of both host models. The early phase of parasitization in BALB/c mice progressed rapidly, with a prepatent period of 7·5–10 days; whereas inM. coucha, the developmental stages ofC. muriswere first observed 12 h later in comparison with BALB/c mice and prepatent period was longer (18–21 days). Similarly, the patent periods of BALB/c mice andM. couchadiffered considerably, i.e. 10–15 daysvschronic infection throughout the life of the host, respectively.