Estimation by gas chromatography of 5β-pregnane-3α, 20α-diol in the urine of normal lactating and ketosed cows

Abstract

SummaryThe measurement by gas chromatography of pregnanediol in the urine of normal and ketosed cows in the first 3 months of lactation is described. Mean pregnanediol levels, corrected for recovery losses, are reported for 3 groups of cows, namely: normal lactating cows 30·7 μg/1 urine; experimentally induced ketosed cows 22·3 μg/1 urine; and primary spontaneously ketosed cows, 31·4 μg/1 urine. No significant differences between the 3 groups of animals were found.