Studies on the growth of marine phytoplankton II. Isochrysis galbana Parke

Abstract

A good deal is known about the growth in culture of Phaeodactylum tricornutum Bohlin (Nitzschia closterium W. Sm. forma minutissima Allen & Nelson) (see Spencer, 1954; Harvey, 1955; Provasoli, McLaughlin & Droop, 1957). This organism, however, is a somewhat aberrant member of the Chrysophyta, and other marine and brackish-water representatives of the group, e.g. Syracosphaera carterae Braarud & Fagerland (Braarud & Fagerland, 1946; Provasoli, McLaughlin & Pintner, 1954), Prymnesium parvum Carter (Reich & Kahn, 1954; Droop, 1954), and certain other species (Droop, 1954, 1955 a, b) have been studied much less intensively from this point of view. It has therefore seemed worth while making a general study, similar to that reported in the first paper of this series for the diatom Asterionella japonica Cleve & Müller ex Gran, of the growth requirements of a representative of the Chrysophyceae. Isochrysis galbana Parke, a flagellate of some importance as a food organism of the oyster, has been selected for this purpose. Johnston (1955) has used unialgal cultures of this species in studies of dissolved organic matter in sea water and, since the investigation being described was carried out, Droop (1957) has reported that it has a requirement for cobalamin.