Genetic characterization of three species ofOnchocercaat 23 enzyme loci

Abstract

ABSTRACTThe technique of allozyme electrophoresis was applied to species ofOnchocercafrom cattle to increase the number of enzyme loci established and therefore provide a genetic basis for a rational species-level taxonomy. Twenty-three enzyme loci were established and provided unequivocal genetic evidence for the taxonomic validity ofOnchocerca gibsoni, O. gutturosaandO. lienalis. Furthermore, the diagnostic enzyme markers detected form the basis for identification of life-cycle stages, individuals and species and population structure analyses.