Immunological Studies on Experimental Infection of Pigs with Ascaris suum Goeze, 1782. II. The Use of the Conglutinating Complement Absorption Test for the Demonstration of Ascaris Antibody

Abstract

The technique of the conglutinating complement absorption test, which has been used to detect antibody in pigs infected with Ascaris, and the method employed for rendering pig serum suitable for use in this test, have been described.Antibodies against Ascaris can be detected by the use of the following antigens: (1) An alcoholic extract of ovaries, oviducts and uteri of the female adult Ascaris. (2) Boiled and unboiled saline extracts of whole worm. (3) Polysaccharide fraction of whole worm.On an examination of antisera from pigs infected with Oesophagostomum dentatiim, no cross-reaction between Oesophagostomum and A. sttum was observed using a saline extract of whole Ascaris worm as antigen.