Author:
Matsuura T.,Bylund G.,Sugane K.
Abstract
ABSTRACTRestriction fragment length polymorphisms (RFLPs) of ribosomal DNA (rDNA) were compared betweenDiphyllobothrium latumandD. nihonkaienseusing seven kinds of restriction endonucleases. No intra-specific variation in restriction fragment profiles was shown within both species ofDiphyllobothrium. Digestion of the genomic DNA with three endonucleases, Smal, Hinfl and Hhal, provided one or two different bands between two species, although the hybridization patterns generated with the others, Hindlll, Xbal, Styl and Haelll, were the same in both. RFLPs in the digested profiles with Smal, Hinfl and Hhal could be used as species-specific markers even if only fragments of strobilae with morphological similarity were available. Other cestodes,Spirometra erinaceiandTaenia saginata, used as controls showed quite different restriction fragment patterns with all the enzymes used.
Publisher
Cambridge University Press (CUP)
Subject
Animal Science and Zoology,General Medicine,Parasitology
Cited by
21 articles.
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