Characterization ofDicrocoelium dendriticumisolates from small ruminants in Shaanxi Province, north-western China, using internal transcribed spacers of nuclear ribosomal DNA

Abstract

AbstractThe genetic variations in internal transcribed spacers (ITS) spanning ITS-1, 5.8S and ITS-2 rDNA ofDicrocoelium dendriticum, isolated from sheep and goats in four geographical regions in Shaanxi province, were examined. The lengths of ITS-1, 5.8S and ITS-2 rDNA sequences forD. dendriticumwere 749 bp, 161 bp and 234 bp, respectively. Intra-specific sequence variations ofD. dendriticumwere 0–0.5% for ITS-1 and 0–1.3% for ITS-2 rDNA, while the inter-specific variations among species in genusDicrocoeliumin ITS-2 rDNA were 3.4–12.3%. Phylogenetic analysis based on sequences of ITS-2 rDNA showed that allD. dendriticumisolates in the present study were grouped with referenceD. dendriticumisolates from sheep and goats, andD. dendriticumisolates from cattle and Japanese serow were clustered in a sister clade. However, the phylogenetic tree could not reveal geographically genetic relationships ofD. dendriticumisolates in different origins and hosts. These findings provided basic information for further study of molecular epidemiology and control ofD. dendriticuminfection in Shaanxi province as well as in the world.