An ensemble of new techniques to study soft-X-ray-induced variations in cellular metabolism-Reference-Cited by-同舟云学术

An ensemble of new techniques to study soft-X-ray-induced variations in cellular metabolism

Published:2004-07 Issue:3 Volume:22 Page:323-333
ISSN:0263-0346
Container-title:Laser and Particle Beams
language:en
Short-container-title:Laser Part. Beams

Author:

TURCU EDMOND,ALLOT RICK,LISI NICOLA,BATANI DIMITRI,BORTOLOTTO FULVIA,MASINI ALESSANDRA,MILANI MARZIALE,BALLERINI MONICA,FERRARO LORENZO,POZZI ACHILLE,PREVIDI FABIO,REBONATO LORENZO

Abstract

An ensemble of new techniques has been developed to study cell metabolism. These include: CO2production monitoring, cell irradiation with soft X rays produced with a laser-plasma source, and study of oscillations in cell metabolic activity via spectral analysis of experimental records. Soft X-rays at about 0.9 keV, with a very low penetration in biological material, were chosen to produce damages at the metabolic level, without great interference with DNA activity. The use of a laser-plasma source allowed a fast deposition of high doses. Monitoring of CO2production allowed us to measure cell metabolic response immediately after irradiation in a continuous and noninvasive way. Also a simple model was developed to calculate X-ray doses delivered to the different cell compartments following a Lambert–Bouguet–Beer law. Results obtained onSaccharomyces cerevisiaeyeast cells in experiments performed at Rutherford Appleton Laboratory are presented.

Publisher

Hindawi Limited

Subject

Electrical and Electronic Engineering,Condensed Matter Physics,Atomic and Molecular Physics, and Optics

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