Intestinal, hepatic, splanchnic and hindquarter amino acid and metabolite partitioning during an establishedTrichostrongylus colubriformisinfection in the small intestine of lambs fed fresh Sulla (Hedysarum coronarium)

Abstract

Increased partitioning of amino acids (AA) from skeletal muscle to the intestine and immune system during parasitic infection may be the cause of poor growth in parasitised animals. The effect of an establishedTrichostrongylus colubriformisinfection (6000 L3T. colubriformislarvae for 6 d (n5) or kept as parasite-free controls (n6)) on AA fluxes across the mesenteric-drained viscera, portal-drained viscera (PDV), liver, total splanchnic tissues (TSP) and hindquarters were determined in lambs fed fresh Sulla (Hedysarum coronarium; 800 g DM/d) 48 d post-infection. The lambs were infused with ρ-aminohippuric acid (PAH; 723 mg/h) into the mesenteric vein for 8 h to measure TSP plasma flow. Concurrently, indocyanine green (ICG; 14·6 mg/h) was infused into the abdominal aorta to measure plasma flow across the hindquarters. Blood was continuously collected from the mesenteric, portal and hepatic veins, vena cava and the mesenteric artery and plasma harvested. PAH, ICG, AA, metabolite and insulin concentrations were measured. Intestinal worm burdens on day 48 post-infection were higher in the infected lambs (P < 0·05). Plasma flows across the tissue beds were unaffected by parasitic infection (P>0·10). There was a 28 % reduction in the release of AA from the PDV of infected lambs (P < 0·05). The uptakes of most AA were similar in the liver; however, there was increased uptake (P < 0·10) of AA by the TSP of infected lambs. Despite this reduction in AA availability at the liver, there was no effect of parasitic infection on AA uptake across the hindquarters (P < 0·05).