Author:
Doran E.,Whittington F. W.,Wood J. D.,McGivan J. D.
Abstract
AbstractThe relationships between the level of skatole in backfat, the rate of skatole metabolism in isolated liver microsomes, hepatic cytochrome P450IIE1 content and mRNA levels were investigated in Large White ✕ Landrace (LW) and the Meishan ✕ Landrace (M) breeds. A method based on thin layer chromatography was developed and used for measurement of microsomal skatole metabolism. Skatole metabolism by liver microsomes was inhibited by diallyl sulphide, a specific inhibitor of cytochrome P450IIE1 but not by inhibitors of other P450 isoforms. We have shown that the rate of skatole metabolism by liver microsomes was proportional to the microsomal P450IIE1 content. In LW pigs there was considerable variation in cytochrome P450IIE1 expression and P450IIE1 protein level and there was a significant negative correlation between backfat skatole level and hepatic microsomal cytochrome P450IIE1 content. Pigs exhibiting low P450IIE1 content in general also showed low levels of P450IIE1 mRNA. These results show that the levels of deposition of backfat skatole in LW pigs are inversely related to the rate at which skatole can be metabolized by liver microsomes, and this in turn depends on the level of expression of cytochrome P450IIE1 mRNA in the liver. In the M breed generally the P450IIE1 protein and mRNA levels were very low and backfat skatole level was high. However the skatole level varied over a 10-fold range and there was no significant correlation with P450IIE1 protein or mRNA content. The M breed was shown to express high levels of the multidrug resistance protein in liver, and it is suggested that export of skatole from liver via this transport protein may be an additional factor regulating backfat skatole in M pigs, but not in the LW breed.
Publisher
Cambridge University Press (CUP)
Subject
Animal Science and Zoology
Reference16 articles.
1. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis;Cleveland;Journal of Biological Chemistry,1977
2. The problem of taint in pork—II. The influence of skatole, androstenone and indole, Presented individually and in combination in a model lipid base, on odour perception
3. [6] Preparation of microsomes with calcium
4. Down-regulation of ras and myc expression associated with mdr-1 overexpression in adriamycin-resistant tumor cells;Banerjee;Cellular and Molecular Biology,1992
5. Induction and tissue-specific expression of rabbit cytochrome P4502E1 and 2E2 genes;Porter;Molecular Pharmacology,1989
Cited by
26 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献