Seasonal carbohydrate fluctuations in hemp dogbane (Apocynum cannabinum) crown roots

Abstract

Field research was conducted to characterize hemp dogbane crown root carbohydrates, to quantify crown root lipids, and to determine seasonal fluctuation of each. The effect of day length on the release of hemp dogbane crown bud dormancy was studied in growth chambers. Total nonstructural carbohydrate (TNC) levels fluctuated with seasonal lows occurring during bud to mid-flowering and seasonal highs in fall predormancy periods. Starch was the primary storage carbohydrate, comprising up to 39.6% of crown root dry weight at the highest seasonal level. Ethanol soluble reducing sugar levels were negatively correlated with seasonal starch levels, and appeared to be maltose and glucose accumulated before conversion to sucrose for translocation. Most lipids were in ethanol soluble fractions and were inversely correlated with seasonal TNC patterns. The maximum level of total lipids in crown roots was 6%. Lipids appeared to be by-products of metabolism in nonarticulated lacticifers and not a significant carbon energy source. An 18-h day length resulted in a 10-fold increase in intact, 2-yr-old crown root dormancy release compared with 10- or 14-h day lengths in growth chamber studies. Seasonal carbohydrate fluctuations in hemp dogbane suggest the most effective control with mowing or tillage would occur when applied at mid- to full flower before root carbohydrates begin to recover. The most effective translocation of phloem mobile herbicides to crown roots would occur after mid- to late flower through leaf senescence when carbohydrates are being stored in the roots.