The Metabolism of Vernolate-14C in Soybean Seedlings

Abstract

The metabolism of S-propyl-1-14C dipropylthiocarbamate (vernolate) was studied in soybean (Glycine max (L.) Merr., var. Lee) seedlings. The degradation of vernlate-14C to 14CO2 was dependent on the age of seedlings. Pretreatment with non-labeled vernolate resulted in a reduction of catabolic oxidation of vernolate-14C to 14CO2, a reduction of cellular incorporation, and a corresponding increase in ethanol soluble metabolites. Paper chromatographic separation of ethanol extracts revealed the presence of two major metabolites and two minor metabolites. The relative abundance of these metabolites was dependent on the age of seedlings and the time of exposure. Results of the time course study suggested the conversion of metabolite 3 to metabolite 4.