Growth Inhibition and Disruption of Mitosis by DCPA in Oat (Avena sativa) Roots

Abstract

One to 5.6 μM DCPA (dimethyl tetrachloroterephthalate) inhibited oat (Avena sativaL. ‘Victory’) root growth within 12 to 18 h. Treated roots were severely stunted and swollen. An analysis of cell division in roots treated with DCPA revealed a disruption of normal mitosis after prophase. Metaphase, anaphase, and telophase division figures were absent 8 to 10 h after treatment with 5.6 μM DCPA. In contrast, a 24-h treatment with 5.6 μM DCPA was necessary to eliminate prophase division figures. The number of aberrant division figures increased concomitantly with the reduction in normal division figures. The predominant type of aberrant division figure was a condensed prophase. When the aberrant division cycle was completed and cells entered interphase, the dispersed chromosomes coalesced to form large, polymorphic nuclei and, occasionally, micronuclei. Approximately 60% of the outer four tiers of cells in roots treated with 5.6 μM DCPA developed abnormal cell walls. These data suggest that DCPA causes root growth inhibition by disrupting several processes involving organized microtubules.