Author:
CHEN J. T.,LIN M. J.,HUANG J. W.
Abstract
SUMMARYPythium damping-off caused byPythium aphanidermatumusually occurs in commercial nurseries of cucumber seedlings. The purpose of the present study was to develop a container medium for effectively suppressing Pythium damping-off of cucumber seedlings. Seven agricultural materials were tested for their suitability as substrates for the growth of cucumber seedlings. Bas Van Burren No. 4 peat moss (BVB) was able to promote the growth of cucumber seedlings, but it was ineffective against Pythium damping-off. Spent blewit mushroom compost (SBMC) was an available substrate that could inhibitP. aphanidermatum, but it also inhibited the growth of cucumber seedlings slightly. A cultural medium, spent blewit peat compost (SBPC), containing 50% (v/v) SBMC, 50% (v/v) BVB and 0·3% (w/v) lime was consequently formulated. The SBPC was able to promote the growth of cucumber seedlings and reduce the incidence of Pythium damping-off. A total of 20 micro-organisms were isolated from SBMC compost and screened in steamed SBPC medium for their ability to inhibit the growth ofP. aphanidermatum. Among these,Bacillus aryabhattaiisolate number CB13 was able to suppressP. aphanidermatumsignificantly in the steamed SBPC medium. Thus, bio-formulation of SBPC medium with the beneficial microbeB. aryabhattaiCB13 was carried out. The incidence of Pythium damping-off was reduced from 58% in steamed SBPC medium to 4% in the bio-formulated container medium. The novel bio-formulation has high potential for controlling Pythium damping-off in commercial nurseries.
Publisher
Cambridge University Press (CUP)
Subject
Genetics,Agronomy and Crop Science,Animal Science and Zoology