Author:
WARPEHA K. M. F.,CAPESIUS I.,GILLILAND T. J.
Abstract
To date, little molecular genetic research has been conducted in
Lolium and the current study is the
first investigation of diversity in ribosomal DNA (rDNA) among perennial
ryegrass cultivars.
Ribosomal DNA probes derived from the intergenic spacer (IGS–M)
and transcribed (COD–M)
regions of mustard (Sinapis alba) were used to examine 35
perennial ryegrass (L. perenne L.) cultivars
for genetic diversity using RFLP methodology. Analysis of
digested genomic DNA on Southern blots
revealed only 10 cultivars with unique RFLP patterns. Genetic
diversity was exclusively associated
with the IGS–M probe, as the cultivars were all
indistinguishable when examined with the COD–M
probe. This low incidence of distinctions was not expected, given
that the 35 cultivars were known
genetically distinct entities, representing the very wide range of the
major phenotypic and
physiological characteristics present in commercial stocks.
Furthermore, the forage cultivars were
proportionately less polymorphic in comparison to the amenity
cultivars. This was also unexpected
as amenity cultivars are recognized as a more closely clustered
group than the forage cultivars, based
on morphological characteristics. The potential use of rDNA for
assessing cultivar diversity and
breeding histories in perennial ryegrass is considered.
Publisher
Cambridge University Press (CUP)
Subject
Genetics,Agronomy and Crop Science,Animal Science and Zoology
Cited by
10 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献