The effect of lactation on protein synthesis in ovine skeletal muscle

Abstract

SUMMARYProtein synthesis was measured in non-breeding sheep and in sheep at two stages of lactation by constant intravenous infusion of [3H] tyrosine. In early lactation plasma tyrosine flux was 50% higher than in non-breeding ewes and it remained somewhat higher in late lactation. Estimates of protein synthesis per day in the whole body showed similar changes.In early lactation the weights of the longissimus dorsi and semitendinosus muscles were respectively 37 and 28% lower than those for non-breeding ewes, but both muscles regained weight in late lactation. There were corresponding changes in the total protein, total RNA and total lipid contents of both muscles.The fractional rates of protein synthesis in the longissimus dorsi and the semitendinosus were between 2 and 3% per day, but it was higher in heart muscle. At both stages of lactation the synthesis rate in the longissimus dorsi was similar to that in nonbreeding ewes, but in the semitendinosus and in the heart synthesis rates were lower in lactating animals. In both skeletal muscles the total protein synthesized per day was lower in early lactation because their total protein contents were lower at this time. It is concluded that the maternal skeletal muscles may undergo a controlled depletion during lactation.