Reproductive and lactational responses and serum growth hormone and insulin in fine-wool ewes treated with ovine growth hormone

Abstract

AbstractEighteen mature (3 to 6 years) Debouillet × Rambouillet ewes (mean weight 79·2 (s.e. 21) kg) producing and nursing single offspring were randomly allotted to one of three groups to examine the influence of ovine growth hormone (oGH) on reproductive, hormonal and lactational responses of springlambing ewes. Each ewe received a daily subcutaneous injection of either 0, 5 or 10 mg oGH from day 11 through to day 20 post partum (day 0 = parturition). Treatment groups were subdivided into two pens (three ewes per pen) and food intake was monitored from day 1 through to day 20 post partum. On days 11 and 20 post partum, jugular blood samples were collected before and hourly for 8 h after treatment. Blood samples were also collected daily from day 10 through to day 21 post partum and on alternate days through to day 40 post partum. Milk production (oxytocin-induced hand milking) and fat and protein concentrations were determined 21 days post partum. Food intake did not differ before or during the treatment period (P > 0·05). Likewise, ewe and lamb weights were similar among treatment groups through to day 60 post partum (P > 0·05). Serum insulin did not differ before or after treatment on day 11 (P > 0·05); however, on day 20 before treatment, serum insulin was significantly higher in ewes receiving 5 (2·3 (s.e. 0·3) μg/I) and 10 (2·4 (s.e. 0·3) μg/1) mg oGH than in controls (1·2 (s.e. 0·3) μg/1) (P < 0·05). After treatment, no differences were detected in serum insulin among treatments on day 20 post partum (P > 0·05). Serum growth hormone (GH) was similar among groups before treatment on day 11 post partum; but by 8 h after treatment, serum GH was significantly elevated in ewes receiving 10 mg oGH (32·4 (s.e. 1·9) μg/1) compared with ewes receiving 5 (13·9 (s.e. 1·9) μg/1) or 0 (4·5 (s.e. 1·9) μg/1) mg oGH (P < 0·01). By 20 days after lambing, serum GH differed significantly among groups both before and after administration of exogenous oGH (P < 0·01). Ewes receiving 5 or 10 mg oGH produced 2·3 (s.e. 0·2) kg milk per day compared with 1·9 (s.e. 0·2) kg/day for controls (P < 0·10). Milk fat and protein concentrations did not differ significantly among treatments (P > 0·05), but total fat yield was higher in ewes receiving either dose of oGH (206 (s.e. 20) g/day) compared with control ewes (141 (s.e. 20) g/day) (P < 005). Serum progesterone remained less than 0·5 μg/1 in ewes through to day 40 post partum. A linear decrease was observed in interval from parturition to first observed oestrus as dosage of oGH increased (116, 108 and 102 (s.e. 6) days for ewes receiving 0, 5 and 10 mg oGH, respectively; P = 0·10). Administration of exogenous oGH to lactating. anoestrous ewes between days 11 and 20 post partum enhances milk production and may influence the interval to oestrus.