Response surface analyses of the effects of dietary protein, fat and carbohydrate on feeding and growth pattern in mice from weaning to maturity

Abstract

AbstractResponses of gross energy intake and body weight of mice from weaning to maturity to dietary composition of energy-yielding nutrients were studied. Twenty-four groups of ddY male mice were fed for 70 days purified diets with a range of 0·08 to 0·75 protein, 0 to 0·92 fat and 0 to 0·92 carbohydrate concentration, calculated on a gross energy basis. Food intake and body weight data in each group were analysed by non-linear regression to obtain values of the parameters in the feeding and growth equation of Parks (1982). Prediction equations for these parameters were obtained with mixture model forms as a function of dietary protein, fat and carbohydrate. These equations for the parameters were then used to construct the response surfaces of body weight and growth rate at a given time on a triangular graph. The response surfaces of body weight at any time were convex, peak positions of which shifted from 043 protein and 0·29 fat at 10 days to 0·36 protein and 0·52 fat at 70 days. The configuration of response surfaces of growth rate (dW/dt) to the three components depends on feeding periods and at the longer periods of feeding the convex configuration of the surfaces became planar. The response surfaces of food efficiency (dW/dF) at any age were almost parallel to those of dW/dt, whereas a parallelism was observed between response surfaces of dW/dt and dF/dt only in young animals. These results suggested that the responses of body weight in young animals to dietary composition of energy-yielding nutrients depended on those of daily energy intake as well as food efficiency, but that those in older animals were affected by food efficiency only.