Author:
Ansari Mohammad Javed,Al-Ghamdi Ahmad,Nuru Adgaba,Ahmed Ashraf Mohamed,Ayaad Tahany H.,Khan Khalid Ali,Al-Waili Noori
Abstract
AbstractApis mellifera jemenitica, the only indigenous honey bee race of Saudi Arabia, is well adapted to the harsh local environmental conditions. A large-scale field survey was conducted to screen major Saudi Arabian beekeeping locations for infection byPaenibacillus larvae. Paenibacillus larvaeis one of the major bacterial pathogens of honey bee broods and is the causative agent of American foulbrood disease. Larvae from samples suspected of infection were collected from different apiaries and homogenized in phosphate-buffered saline. Bacteria were isolated on MYPGP agar medium. Two bacterial isolates, ksuPL3 and ksuPL5 (16S rRNA GenBank accession numbers, KR780760 and KR780761, respectively), were subjected to molecular identification usingP. larvae-specific primers. A BLAST sequence analysis revealed that the two isolates wereP. larvaewith more than 98% sequence identity. This detection ofP. larvaein the indigenous honey bee is the first recorded incidence of this pathogen in Saudi Arabia. This study emphasizes the need for the relevant authorities to take immediate steps towards treating and limiting the spread of this disease throughout the country.
Publisher
Springer Science and Business Media LLC
Subject
Insect Science,Ecology, Evolution, Behavior and Systematics
Cited by
10 articles.
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