V.—The Mode of Multiplication of the Rous No. I Sarcoma Virus

Abstract

SynopsisKnown amounts of the Rous No. 1 sarcoma virus were injected into the legs of chicks. The birds were killed, the legs extracted, and the virus content determined at intervals. Controls showed complete recovery of the virus by the methods used. After 16 hours the amount of virus was 1 per cent, or less of the injected amount, and this low level remained until about 70 hours, when an increased amount of virus suddenly appeared. This is taken to imply that, like the necrotising viruses, those of the fowl sarcoma multiply in the cell in a non-infective and probably disaggregated form. These times agree well with previous histological findings. The implications of such a method of reproduction are discussed.