Mouse testicular protein variants, including tubulin, detected by isoelectrofocusing two-dimensional gel electrophoresis

Abstract

SummaryWe have utilized isoelectrofocusing two-dimensional polyacrylamide gel electrophoresis (IEF-ID-PAGE) to determine the frequency of variant proteins detected in mouse testes, and to examine an inbred strain with spermatogenic abnormalities. The observed frequency of variants, 4·2 ± 1·0%, is comparable to results reported for liver. A variant for a protein, identified as tubulin by immunoblotting, was observed in an inbred strain (PL/J) which has defective spermatogenesis, but was also observed in another inbred strain without spermatogenic defects. The gene symbolTbnis proposed for this β-tubulin locus.