Heterozygous diploid analyses via the parasexual cycle and a cytoplasmic pattern of inheritance inVerticillium spp.

Abstract

Combining data from mitotic crossing over in, and haploidization of, heterozygous diploid strains, 33 markers have been mapped successfully inVerticilliumspp. One marker (Hyl), which determines the absence of darkly pigmented resting structures in many stable hyaline strains, showed an irregular segregation. In view of previous evidence from acriflavine treatment and heterokaryon analyses in the fungus, this further substantiated indications of a cytoplasmic pattern of inheritance for this marker. The phenotypic expression of dark pigment genes showing nuclear inheritance, such asSot., depends upon the cytoplasmically determined (Hyl+) presence of resting structures.