Isolation ofBacillus subtilistransformation-deficient mutants and mapping of competence genes-Reference-Cited by-同舟云学术

Isolation ofBacillus subtilistransformation-deficient mutants and mapping of competence genes

Published:1989-08 Issue:1 Volume:54 Page:1-6
ISSN:0016-6723
Container-title:Genetical Research
language:en
Short-container-title:Genet. Res.

Author:

Mastromei Giorgio,Barberio Claudia,Pistolesi Stefania,Polsinelli Mario

Abstract

SummaryWe have isolated and characterized 48Bacillus subtiliscompetence-deficient mutants. The mutants, obtained by nitrososoguanidine mutagenesis or by insertional mutagenesis with transposon Tn917, had a reduced transformation frequency and a wild-type transduction frequency. Thecommutations were mapped by PBS1 transduction and at least four newcomgenes have been identified. The mutants were also characterized for their capacity to bind and take up the transforming DNA.

Publisher

Hindawi Limited

Subject

Genetics,General Medicine

Reference22 articles.

1. Isolation and partial characterization of Bacillus subtilis mutants impaired in DNA entry;Mulder;Journal of Bacteriology,1982

2. Isolation and characterization of Bacillus subtilis mutants altered in competence;Fani;Journal of Bacteriology,1984

3. Transformation in Bacillus subtilis: properties of DNA binding deficient mutants;Smith;Journal of Bacteriology,1983

4. Transformation in Bacillus subtilis: further characterization of a 75,000 dalton protein complex involved in binding and entry of donor DNA;Smith;Journal of Bacteriology,1985

5. Genetic transposition and insertional mutagenesis in Bacillus subtilis with Streptococcus faecalis transposon Tn917.

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