Author:
PARKS K. R.,EISEN E. J.,PARKER I. J.,HESTER L. G.,MURRAY J. D.
Abstract
Correlated responses in female reproductive performance were evaluated following short-term
selection within full-sib families for increased 8-week body weight in two replicates of four lines of
mice: two ovine metallothionein–ovine growth hormone (oMt1a-oGH) transgene-carrier lines, one
from a high-growth background (TM) and one from a control background (TC), and two non-transgenic lines, one from each of these genetic backgrounds (NM and NC, respectively). A fifth
line (CC), not containing the transgene, served as a randomly selected control. The initial
frequency of the oMt1a-oGH transgene construct in the TM and TC lines was 0·5. The frequency
of transgenic females sampled at generations 7 and 8 of selection was 84·0% and 6·1% in the TC
and TM lines, respectively. No significant female infertility differences were detected between
transgene-carrier and non-transgenic lines or between transgenic and non-transgenic mice within
carrier lines, whereas high-growth background lines had a higher infertility than control
background lines (P < 0·05). Correlated responses in the TC transgene-carrier line were suggestive
of reduced reproductive performance as indicated by increased post-implantation mortality
(P < 0·05), number of dead fetuses plus implants (P < 0·05), and loss of fetuses from day 16 to
parturition (P < 0·001). For the first two traits, the negative correlated responses were accounted
for by the reduced performance of transgenic compared with non-transgenic females. Embryos
carrying the transgene may also have a lower viability. In contrast, the NC non-transgenic line did
not exhibit reduced reproductive performance for these traits. The low frequency of the transgene
in the high-growth background TM line was associated with reduced fitness and a lower additive
effect for 8-week body weight compared with the control background TC line.
Subject
Genetics,General Medicine
Cited by
4 articles.
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