In vitrobiological control of bovine parasitic nematodes byArthrobotrys cladodes,Duddingtonia flagransandPochonia chlamydosporiaunder different temperature conditions

Abstract

AbstractVariations in temperature can affect the development of nematophagous fungi, especially when they are used in the biological control of parasitic nematodes in the pastures where cattle are reared. The aim of this work was to evaluate the effects of temperature on the performance of nematophagous fungi in the biological control of bovine parasitic nematodes. The mycelial growth, chlamydospore production and nematicidal activity ofDuddingtonia flagrans, Arthrobotrys cladodesandPochonia chlamydosporiawere evaluated at 15, 20, 25, 30 and 35°C. The fungal strains achieved mycelial growth, chlamydospore production and nematicidal activity on parasitic nematodes under all temperature conditions tested. The fungi showed higher growth at intermediate temperatures (20, 25 and 30°C) than at the extremes of 15 and 35°C. At 25 and 30°C,D. flagransrealized 96.8 and 94.5% nematicidal activity on bovine parasitic nematodes, respectively.Arthrobotrys cladodeseffected nematicidal activity of 85.3 and 83.5%, at 20 and 25°C, respectively. At 20 and 30°C,P. chlamydosporiaachieved nematicidal activity of 81.3 and 87.4%, respectively. The maximum chlamydospore production was reached at 20, 25 and 30°C forD. flagrans, at 20 and 25°C forA. cladodesandP. chlamydosporia.The results of this study demonstrated that the tested fungal strains ofD. flagrans, A. cladodesandP. chlamydosporia,when used in the biological control of bovine parasitic nematodes, were not limited byin vitrotemperature variations. Therefore, the use of these strains of fungi as biological control agents of parasitic nematodes is promising.