Histochemical localization and functional significance of some enzymes in various tissues ofCeylonocotyle scoliocoeliumcultured invitro

Abstract

ABSTRACTTPase and SDII activities were studied in various tissues ofCeylonocotyle scoliocoeliummaintained invitroin medium A for 15 days, using standard histochemical techniques. Results were compared with the normal activities of these enzymes in the tissues of worms freshly collected. Low ATPase and SDH enzyme activities in the tissues of cultured flukes indicated their low metabolic rate. Intensity of enzymatic reactions was also found to be tissue specific. The suitability of culture medium ‘A’ for the invitrocultivation of amphistomes is discussed.