Compartmental flux andin situmethods underestimate total feed nitrogen as judged by the omasal sampling method due to ignoring soluble feed nitrogen flow

Abstract

The objective of the present study was to estimate ruminal feed N outflow in lactating cows using the omasal sampling, compartmental flux orin situmethod. A total of five ruminally fistulated Finnish Ayrshire dairy cows were used in a 5 × 5 Latin square study with 21 d periods. Experimental silages of grass or red clover harvested at two stages of maturity in addition to a supplement of 9·0 kg concentrate/d were fed to the cows.In vivoomasal N flow was determined using the omasal sampling technique. Ruminalin situN flow was calculated from N intake and degradability (38 μm nylon bags). The samples of ruminal contents and faeces were divided into seven particle-size fractions by wet sieving; the concentrations of indigestible neutral-detergent fibre and N were used to calculate N flow in the compartmental flux method.In vivoomasal N flow was greater for the red clover silage diets than for the grass silage diets. The N flow calculated using the compartmental flux technique and that calculated using thein situtechnique were highly correlated, but both were less than and poorly correlated with thein vivoN flow. In bothin situand compartmental flux techniques, forage maturity increased the particle-associated N flow, with the increase being significantly greater for the red clover diets than for the grass silage diets. In conclusion, the compartmental flux andin situmethods described the N flow associated with the particle fractions rather than the total ruminal outflow of feed N.