Interactions of ethanol drinking withn-3 fatty acids in rats: potential consequences for the cardiovascular system

Abstract

Moderate ethanol drinking (ED) andn-3 fatty acids have both been associated with low cardiac mortality. However, there are few data evaluating the interactions of ED withn-3. We recently reported that moderate ED results in increasedn-3 in cardiac patients. The main aim of the present study was, through a well-controlled experimental model, to confirm that chronic ED actually results in increasedn-3. Secondary aims were to examine the effects of chronic ED on cardiac mitochondria, cardiac function and experimental myocardial infarction. We studied the fatty acid profiles of plasma, cell membranes and cardiac mitochondria phospholipids in a rat model of chronic ED. In plasma and cell membranes, ED actually resulted in highern-3 (P = 0·005). In mitochondria phospholipids of ED rats,n-3 were also increased (P < 0·05) but quite modestly. Cardiac mitochondrial function and left ventricular function were not significantly different in ED and control rats, while infarct size after 30 min ischaemia and reperfusion was smaller (P < 0·0001) in ED rats. This is the first animal study confirming interaction of alcohol drinking withn-3. We found no harmful effect of chronic ED on the heart in that model but a significant cardioprotection. Further studies are warranted to investigate the mechanisms by which moderate ED alters the metabolism ofn-3 and whethern-3 are the mediators of the ED-induced cardioprotection.